The Ino80 complex mediates epigenetic centromere propagation via active removal of histone H3

Eun Shik Choi, Youngseo Cheon, Keunsoo Kang and Daeyoup Lee ()
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Eun Shik Choi: Korea Advanced Institute of Science and Technology
Youngseo Cheon: Korea Advanced Institute of Science and Technology
Keunsoo Kang: Dankook University
Daeyoup Lee: Korea Advanced Institute of Science and Technology

Nature Communications, 2017, vol. 8, issue 1, 1-10

Abstract: Abstract The centromere is the chromosomal locus at which the kinetochore is assembled to direct chromosome segregation. The histone H3 variant, centromere protein A (CENP-A), is known to epigenetically mark active centromeres, but the mechanism by which CENP-A propagates at the centromere, replacing histone H3, remains poorly understood. Using fission yeast, here we show that the Ino80 adenosine triphosphate (ATP)-dependent chromatin-remodeling complex, which removes histone H3-containing nucleosomes from associated chromatin, promotes CENP-ACnp1 chromatin assembly at the centromere in a redundant manner with another chromatin-remodeling factor Chd1Hrp1. CENP-ACnp1 chromatin actively recruits the Ino80 complex to centromeres to elicit eviction of histone H3-containing nucleosomes. Artificial targeting of Ino80 subunits to a non-centromeric DNA sequence placed in a native centromere enhances the spreading of CENP-ACnp1 chromatin into the non-centromeric DNA. Based on these results, we propose that CENP-ACnp1 chromatin employs the Ino80 complex to mediate the replacement of histone H3 with CENP-ACnp1, and thereby reinforces itself.

Date: 2017
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DOI: 10.1038/s41467-017-00704-3

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