Transcriptional regulation of endothelial cell behavior during sprouting angiogenesis

Hyun-Woo Jeong, Benjamín Hernández-Rodríguez, JungMo Kim, Kee-Pyo Kim, Rocio Enriquez-Gasca, Juyong Yoon, Susanne Adams, Hans R. Schöler, Juan M. Vaquerizas () and Ralf H. Adams ()
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Hyun-Woo Jeong: Max-Planck-Institute for Molecular Biomedicine
Benjamín Hernández-Rodríguez: Max-Planck-Institute for Molecular Biomedicine
JungMo Kim: Max-Planck-Institute for Molecular Biomedicine
Kee-Pyo Kim: Max-Planck-Institute for Molecular Biomedicine
Rocio Enriquez-Gasca: Max-Planck-Institute for Molecular Biomedicine
Juyong Yoon: Max-Planck-Institute for Molecular Biomedicine
Susanne Adams: Max-Planck-Institute for Molecular Biomedicine
Hans R. Schöler: University of Münster
Juan M. Vaquerizas: Max-Planck-Institute for Molecular Biomedicine
Ralf H. Adams: Max-Planck-Institute for Molecular Biomedicine

Nature Communications, 2017, vol. 8, issue 1, 1-14

Abstract: Abstract Mediating the expansion of vascular beds in many physiological and pathological settings, angiogenesis requires dynamic changes in endothelial cell behavior. However, the molecular mechanisms governing endothelial cell activity during different phases of vascular growth, remodeling, maturation, and quiescence remain elusive. Here, we characterize dynamic gene expression changes during postnatal development and identify critical angiogenic factors in mouse retinal endothelial cells. Using actively translating transcriptome analysis and in silico computational analyses, we determine candidate regulators controlling endothelial cell behavior at different developmental stages. We further show that one of the identified candidates, the transcription factor MafB, controls endothelial sprouting in vitro and in vivo, and perform an integrative analysis of RNA-Seq and ChIP-Seq data to define putative direct MafB targets, which are activated or repressed by the transcriptional regulator. Together, our results identify novel cell-autonomous regulatory mechanisms controlling sprouting angiogenesis.

Date: 2017
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-00738-7

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DOI: 10.1038/s41467-017-00738-7

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