High-throughput RNA structure probing reveals critical folding events during early 60S ribosome assembly in yeast

Elena Burlacu, Fredrik Lackmann, Lisbeth-Carolina Aguilar, Sergey Belikov, Rob van Nues, Christian Trahan, Ralph D. Hector, Nicholas Dominelli-Whiteley, Scott L. Cockroft, Lars Wieslander, Marlene Oeffinger and Sander Granneman ()
Additional contact information
Elena Burlacu: University of Edinburgh
Fredrik Lackmann: The Wenner-Gren Institute, Stockholm University
Lisbeth-Carolina Aguilar: Systems Biology Axis
Sergey Belikov: The Wenner-Gren Institute, Stockholm University
Rob van Nues: Institute of Cell Biology, University of Edinburgh
Christian Trahan: Systems Biology Axis
Ralph D. Hector: Institute of Neuroscience and Psychology, University of Glasgow
Nicholas Dominelli-Whiteley: EaStCHEM School of Chemistry, University of Edinburgh
Scott L. Cockroft: EaStCHEM School of Chemistry, University of Edinburgh
Lars Wieslander: The Wenner-Gren Institute, Stockholm University
Marlene Oeffinger: Systems Biology Axis
Sander Granneman: University of Edinburgh

Nature Communications, 2017, vol. 8, issue 1, 1-14

Abstract: Abstract While the protein composition of various yeast 60S ribosomal subunit assembly intermediates has been studied in detail, little is known about ribosomal RNA (rRNA) structural rearrangements that take place during early 60S assembly steps. Using a high-throughput RNA structure probing method, we provide nucleotide resolution insights into rRNA structural rearrangements during nucleolar 60S assembly. Our results suggest that many rRNA-folding steps, such as folding of 5.8S rRNA, occur at a very specific stage of assembly, and propose that downstream nuclear assembly events can only continue once 5.8S folding has been completed. Our maps of nucleotide flexibility enable making predictions about the establishment of protein–rRNA interactions, providing intriguing insights into the temporal order of protein–rRNA as well as long-range inter-domain rRNA interactions. These data argue that many distant domains in the rRNA can assemble simultaneously during early 60S assembly and underscore the enormous complexity of 60S synthesis.

Date: 2017
References: Add references at CitEc
Citations: View citations in EconPapers (2)

Downloads: (external link)
https://www.nature.com/articles/s41467-017-00761-8 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-00761-8

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-017-00761-8

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().