PD-L1 is an activation-independent marker of brown adipocytes

Jessica R. Ingram, Michael Dougan, Mohammad Rashidian, Marko Knoll, Edmund J. Keliher, Sarah Garrett, Scott Garforth, Olga S. Blomberg, Camilo Espinosa, Atul Bhan, Steven C. Almo, Ralph Weissleder, Harvey Lodish, Stephanie K. Dougan and Hidde L. Ploegh ()
Additional contact information
Jessica R. Ingram: Whitehead Institute for Biomedical Research
Michael Dougan: Whitehead Institute for Biomedical Research
Mohammad Rashidian: Whitehead Institute for Biomedical Research
Marko Knoll: Whitehead Institute for Biomedical Research
Edmund J. Keliher: Massachusetts General Hospital
Sarah Garrett: Albert Einstein College of Medicine
Scott Garforth: Albert Einstein College of Medicine
Olga S. Blomberg: Whitehead Institute for Biomedical Research
Camilo Espinosa: Whitehead Institute for Biomedical Research
Atul Bhan: Massachusetts General Hospital
Steven C. Almo: Albert Einstein College of Medicine
Ralph Weissleder: Massachusetts General Hospital
Harvey Lodish: Whitehead Institute for Biomedical Research
Stephanie K. Dougan: Dana-Farber Cancer Institute
Hidde L. Ploegh: Whitehead Institute for Biomedical Research

Nature Communications, 2017, vol. 8, issue 1, 1-10

Abstract: Abstract Programmed death ligand 1 (PD-L1) is expressed on a number of immune and cancer cells, where it can downregulate antitumor immune responses. Its expression has been linked to metabolic changes in these cells. Here we develop a radiolabeled camelid single-domain antibody (anti-PD-L1 VHH) to track PD-L1 expression by immuno-positron emission tomography (PET). PET-CT imaging shows a robust and specific PD-L1 signal in brown adipose tissue (BAT). We confirm expression of PD-L1 on brown adipocytes and demonstrate that signal intensity does not change in response to cold exposure or β-adrenergic activation. This is the first robust method of visualizing murine brown fat independent of its activation state.

Date: 2017
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DOI: 10.1038/s41467-017-00799-8

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