MicroRNA-9 downregulates the ANO1 chloride channel and contributes to cystic fibrosis lung pathology

Florence Sonneville, Manon Ruffin, Christelle Coraux, Nathalie Rousselet, Philippe Le Rouzic, Sabine Blouquit-Laye, Harriet Corvol and Olivier Tabary ()
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Florence Sonneville: Sorbonne Universités, UPMC Univ Paris 06
Manon Ruffin: Sorbonne Universités, UPMC Univ Paris 06
Christelle Coraux: University of Reims Champagne-Ardenne
Nathalie Rousselet: Sorbonne Universités, UPMC Univ Paris 06
Philippe Le Rouzic: Sorbonne Universités, UPMC Univ Paris 06
Sabine Blouquit-Laye: Université de Versailles Saint Quentin en Yvelines, UFR des Sciences de la Santé
Harriet Corvol: Sorbonne Universités, UPMC Univ Paris 06
Olivier Tabary: Sorbonne Universités, UPMC Univ Paris 06

Nature Communications, 2017, vol. 8, issue 1, 1-11

Abstract: Abstract Cystic fibrosis results from reduced cystic fibrosis transmembrane conductance regulator protein activity leading to defective epithelial ion transport. Ca2+-activated Cl− channels mediate physiological functions independently of cystic fibrosis transmembrane conductance regulator. Anoctamin 1 (ANO1/TMEM16A) was identified as the major Ca2+-activated Cl− channel in airway epithelial cells, and we recently demonstrated that downregulation of the anoctamin 1 channel in cystic fibrosis patients contributes to disease severity via an unknown mechanism. Here we show that microRNA-9 (miR-9) contributes to cystic fibrosis and downregulates anoctamin 1 by directly targeting its 3′UTR. We present a potential therapy based on blockage of miR-9 binding to the 3′UTR by using a microRNA target site blocker to increase anoctamin 1 activity and thus compensate for the cystic fibrosis transmembrane conductance regulator deficiency. The target site blocker is tested in in vitro and in mouse models of cystic fibrosis, and could be considered as an alternative strategy to treat cystic fibrosis.

Date: 2017
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DOI: 10.1038/s41467-017-00813-z

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