PNLDC1 is essential for piRNA 3′ end trimming and transposon silencing during spermatogenesis in mice

Deqiang Ding, Jiali Liu, Kunzhe Dong, Uros Midic, Rex A. Hess, Huirong Xie, Elena Y. Demireva and Chen Chen ()
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Deqiang Ding: Michigan State University
Jiali Liu: Michigan State University
Kunzhe Dong: Avian Disease and Oncology Laboratory
Uros Midic: Michigan State University
Rex A. Hess: University of Illinois
Huirong Xie: Michigan State University
Elena Y. Demireva: Michigan State University
Chen Chen: Michigan State University

Nature Communications, 2017, vol. 8, issue 1, 1-10

Abstract: Abstract Piwi-interacting RNAs are small regulatory RNAs with key roles in transposon silencing and regulation of gametogenesis. The production of mature piwi-interacting RNAs requires a critical step of trimming piwi-interacting RNA intermediates to achieve optimally sized piwi-interacting RNAs. The poly(A)-specific ribonuclease family deadenylase PNLDC1 is implicated in piwi-interacting RNA trimming in silkworms. The physiological function of PNLDC1 in mammals remains unknown. Using Pnldc1-deficient mice, here we show that PNLDC1 is required for piwi-interacting RNA biogenesis, transposon silencing, and spermatogenesis. Pnldc1 mutation in mice inhibits piwi-interacting RNA trimming and causes accumulation of untrimmed piwi-interacting RNA intermediates with 3′ end extension, leading to severe reduction of mature piwi-interacting RNAs in the testis. Pnldc1 mutant mice exhibit disrupted LINE1 retrotransposon silencing and defect in spermiogenesis. Together, these results define PNLDC1 as a mammalian piwi-interacting RNA biogenesis factor that protects the germline genome and ensures normal sperm production in mice.

Date: 2017
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DOI: 10.1038/s41467-017-00854-4

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