Characterization of a dual function macrocyclase enables design and use of efficient macrocyclization substrates

Clarissa M. Czekster, Hannes Ludewig, Stephen A. McMahon and James H. Naismith ()
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Clarissa M. Czekster: The University of St Andrews, North Haugh
Hannes Ludewig: The University of St Andrews, North Haugh
Stephen A. McMahon: The University of St Andrews, North Haugh
James H. Naismith: The University of St Andrews, North Haugh

Nature Communications, 2017, vol. 8, issue 1, 1-10

Abstract: Abstract Peptide macrocycles are promising therapeutic molecules because they are protease resistant, structurally rigid, membrane permeable, and capable of modulating protein–protein interactions. Here, we report the characterization of the dual function macrocyclase-peptidase enzyme involved in the biosynthesis of the highly toxic amanitin toxin family of macrocycles. The enzyme first removes 10 residues from the N-terminus of a 35-residue substrate. Conformational trapping of the 25 amino-acid peptide forces the enzyme to release this intermediate rather than proceed to macrocyclization. The enzyme rebinds the 25 amino-acid peptide in a different conformation and catalyzes macrocyclization of the N-terminal eight residues. Structures of the enzyme bound to both substrates and biophysical analysis characterize the different binding modes rationalizing the mechanism. Using these insights simpler substrates with only five C-terminal residues were designed, allowing the enzyme to be more effectively exploited in biotechnology.

Date: 2017
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DOI: 10.1038/s41467-017-00862-4

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