Single nucleus sequencing reveals spermatid chromosome fragmentation as a possible cause of maize haploid induction

Xiang Li, Dexuan Meng, Shaojiang Chen, Haishan Luo, Qinghua Zhang, Weiwei Jin () and Jianbing Yan ()
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Xiang Li: Huazhong Agricultural University
Dexuan Meng: China Agricultural University
Shaojiang Chen: China Agricultural University
Haishan Luo: China Agricultural University
Qinghua Zhang: Huazhong Agricultural University
Weiwei Jin: China Agricultural University
Jianbing Yan: Huazhong Agricultural University

Nature Communications, 2017, vol. 8, issue 1, 1-9

Abstract: Abstract Production of maternal haploids using a conspecific haploid inducer is routine and highly efficient in maize. However, the underlying mechanism of haploid induction (HI) is unclear. We develop a method to isolate three nuclei from a pollen grain and four microspores from a tetrad for whole-genome sequencing. A high rate of aneuploidy is observed at the three-nucleus stage (6/22 pollens) rather than at the tetrad stage (1/72 microspores) in one HI line CAU5. Frequent aneuploidy is also observed in another two inducer lines, but not in two regular lines, which implies that HI may be associated with pollen aneuploidy. We further sequence the individual embryos and endosperms of 88 maize kernels crossing between regular and inducer lines. Genome-wide elimination of the CAU5-derived chromosome is identified in eight of 81 embryos. Together, these results suggest that continuous chromosome fragmentation occurring post meiosis in the gametophyte may cause haploidy of the embryo.

Date: 2017
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DOI: 10.1038/s41467-017-00969-8

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