Characteristic rotational behaviors of rod-shaped cargo revealed by automated five-dimensional single particle tracking

Kuangcai Chen, Yan Gu, Wei Sun, Dong, Gufeng Wang, Xinxin Fan, Tian Xia () and Ning Fang ()
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Kuangcai Chen: Georgia State University
Yan Gu: Iowa State University
Wei Sun: Iowa State University
Dong: Georgia State University
Gufeng Wang: Iowa State University
Xinxin Fan: Huazhong University of Science and Technology
Tian Xia: Huazhong University of Science and Technology
Ning Fang: Georgia State University

Nature Communications, 2017, vol. 8, issue 1, 1-10

Abstract: Abstract We report an automated single particle tracking technique for tracking the x, y, z coordinates, azimuthal and elevation angles of anisotropic plasmonic gold nanorod probes in live cells. These five spatial coordinates are collectively referred to as 5D. This method overcomes a long-standing challenge in distinguishing rotational motions from translational motions in the z-axis in differential interference contrast microscopy to result in full disclosure of nanoscale motions with high accuracy. Transferrin-coated endocytic gold nanorod cargoes initially undergo active rotational diffusion and display characteristic rotational motions on the membrane. Then as the cargoes being enclosed in clathrin-coated pits, they slow down the active rotation and experience a quiet period before they restore active rotational diffusion after fission and eventually being transported away from the original entry spots. Finally, the 3D trajectories and the accompanying rotational motions of the cargoes are resolved accurately to render the intracellular transport process in live cells.

Date: 2017
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DOI: 10.1038/s41467-017-01001-9

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