Electro-osmotic capture and ionic discrimination of peptide and protein biomarkers with FraC nanopores

Gang Huang, Kherim Willems, Misha Soskine, Carsten Wloka () and Giovanni Maglia ()
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Gang Huang: University of Groningen
Kherim Willems: KU Leuven Department of Chemistry, Celestijnenlaan 200G
Misha Soskine: University of Groningen
Carsten Wloka: University of Groningen
Giovanni Maglia: University of Groningen

Nature Communications, 2017, vol. 8, issue 1, 1-11

Abstract: Abstract Biological nanopores are nanoscale sensors employed for high-throughput, low-cost, and long read-length DNA sequencing applications. The analysis and sequencing of proteins, however, is complicated by their folded structure and non-uniform charge. Here we show that an electro-osmotic flow through Fragaceatoxin C (FraC) nanopores can be engineered to allow the entry of polypeptides at a fixed potential regardless of the charge composition of the polypeptide. We further use the nanopore currents to discriminate peptide and protein biomarkers from 25 kDa down to 1.2 kDa including polypeptides differing by one amino acid. On the road to nanopore proteomics, our findings represent a rationale for amino-acid analysis of folded and unfolded polypeptides with nanopores.

Date: 2017
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DOI: 10.1038/s41467-017-01006-4

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