Single-cell mass cytometry and transcriptome profiling reveal the impact of graphene on human immune cells

Marco Orecchioni, Davide Bedognetti, Leon Newman, Claudia Fuoco, Filomena Spada, Wouter Hendrickx, Francesco M. Marincola, Francesco Sgarrella, Artur Filipe Rodrigues, Cécilia Ménard-Moyon, Gianni Cesareni, Kostas Kostarelos (), Alberto Bianco () and Lucia G Delogu ()
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Marco Orecchioni: Department of Chemistry and Pharmacy University of Sassari
Davide Bedognetti: Sidra Medical and Research Center
Leon Newman: Faculty of Biology, Medicine & Health, and National Graphene Institute University of Manchester
Claudia Fuoco: University of Rome Tor Vergata
Filomena Spada: University of Rome Tor Vergata
Wouter Hendrickx: Sidra Medical and Research Center
Francesco M. Marincola: Sidra Medical and Research Center
Francesco Sgarrella: Department of Chemistry and Pharmacy University of Sassari
Artur Filipe Rodrigues: Faculty of Biology, Medicine & Health, and National Graphene Institute University of Manchester
Cécilia Ménard-Moyon: Laboratoire d’Immunopathologie et Chimie Thérapeutique
Gianni Cesareni: University of Rome Tor Vergata
Kostas Kostarelos: Faculty of Biology, Medicine & Health, and National Graphene Institute University of Manchester
Alberto Bianco: Laboratoire d’Immunopathologie et Chimie Thérapeutique
Lucia G Delogu: Department of Chemistry and Pharmacy University of Sassari

Nature Communications, 2017, vol. 8, issue 1, 1-14

Abstract: Abstract Understanding the biomolecular interactions between graphene and human immune cells is a prerequisite for its utilization as a diagnostic or therapeutic tool. To characterize the complex interactions between graphene and immune cells, we propose an integrative analytical pipeline encompassing the evaluation of molecular and cellular parameters. Herein, we use single-cell mass cytometry to dissect the effects of graphene oxide (GO) and GO functionalized with amino groups (GONH2) on 15 immune cell populations, interrogating 30 markers at the single-cell level. Next, the integration of single-cell mass cytometry with genome-wide transcriptome analysis shows that the amine groups reduce the perturbations caused by GO on cell metabolism and increase biocompatibility. Moreover, GONH2 polarizes T-cell and monocyte activation toward a T helper-1/M1 immune response. This study describes an innovative approach for the analysis of the effects of nanomaterials on distinct immune cells, laying the foundation for the incorporation of single-cell mass cytometry on the experimental pipeline.

Date: 2017
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DOI: 10.1038/s41467-017-01015-3

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