Control of enzyme reactions by a reconfigurable DNA nanovault

Guido Grossi, Mette Dalgaard Ebbesen Jepsen, Jørgen Kjems and Ebbe Sloth Andersen ()
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Guido Grossi: Aarhus University
Mette Dalgaard Ebbesen Jepsen: Aarhus University
Jørgen Kjems: Aarhus University
Ebbe Sloth Andersen: Aarhus University

Nature Communications, 2017, vol. 8, issue 1, 1-8

Abstract: Abstract Biological systems use compartmentalisation as a general strategy to control enzymatic reactions by precisely regulating enzyme–substrate interactions. With the advent of DNA nanotechnology, it has become possible to rationally design DNA-based nano-containers with programmable structural and dynamic properties. These DNA nanostructures have been used to cage enzymes, but control over enzyme–substrate interactions using a dynamic DNA nanostructure has not been achieved yet. Here we introduce a DNA origami device that functions as a nanoscale vault: an enzyme is loaded in an isolated cavity and the access to free substrate molecules is controlled by a multi-lock mechanism. The DNA vault is characterised for features such as reversible opening/closing, cargo loading and wall porosity, and is shown to control the enzymatic reaction catalysed by an encapsulated protease. The DNA vault represents a general concept to control enzyme–substrate interactions by inducing conformational changes in a rationally designed DNA nanodevice.

Date: 2017
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DOI: 10.1038/s41467-017-01072-8

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