Mass spectrometry sequencing of long digital polymers facilitated by programmed inter-byte fragmentation

Abdelaziz Al Ouahabi, Jean-Arthur Amalian, Laurence Charles () and Jean-François Lutz ()
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Abdelaziz Al Ouahabi: Institut Charles Sadron UPR22
Jean-Arthur Amalian: Institute of Radical Chemistry
Laurence Charles: Institute of Radical Chemistry
Jean-François Lutz: Institut Charles Sadron UPR22

Nature Communications, 2017, vol. 8, issue 1, 1-8

Abstract: Abstract In the context of data storage miniaturization, it was recently shown that digital information can be stored in the monomer sequences of non-natural macromolecules. However, the sequencing of such digital polymers is currently limited to short chains. Here, we report that intact multi-byte digital polymers can be sequenced in a moderate resolution mass spectrometer and that full sequence coverage can be attained without requiring pre-analysis digestion or the help of sequence databases. In order to do so, the polymers are designed to undergo controlled fragmentations in collision-induced dissociation conditions. Each byte of the sequence is labeled by an identification tag and a weak alkoxyamine group is placed between 2 bytes. As a consequence of this design, the NO-C bonds break first upon collisional activation, thus leading to a pattern of mass tag-shifted intact bytes. Afterwards, each byte is individually sequenced in pseudo-MS3 conditions and the whole sequence is found.

Date: 2017
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DOI: 10.1038/s41467-017-01104-3

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