Achieving high-efficiency emission depletion nanoscopy by employing cross relaxation in upconversion nanoparticles

Qiuqiang Zhan (), Haichun Liu, Baoju Wang, Qiusheng Wu, Rui Pu, Chao Zhou, Bingru Huang, Xingyun Peng, Hans Ågren and Sailing He ()
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Qiuqiang Zhan: South China Academy of Advanced Optoelectronics, South China Normal University
Haichun Liu: Royal Institute of Technology
Baoju Wang: South China Academy of Advanced Optoelectronics, South China Normal University
Qiusheng Wu: South China Academy of Advanced Optoelectronics, South China Normal University
Rui Pu: South China Academy of Advanced Optoelectronics, South China Normal University
Chao Zhou: South China Academy of Advanced Optoelectronics, South China Normal University
Bingru Huang: South China Academy of Advanced Optoelectronics, South China Normal University
Xingyun Peng: South China Academy of Advanced Optoelectronics, South China Normal University
Hans Ågren: Royal Institute of Technology
Sailing He: South China Academy of Advanced Optoelectronics, South China Normal University

Nature Communications, 2017, vol. 8, issue 1, 1-11

Abstract: Abstract Stimulated emission depletion microscopy provides a powerful sub-diffraction imaging modality for life science studies. Conventionally, stimulated emission depletion requires a relatively high light intensity to obtain an adequate depletion efficiency through only light–matter interaction. Here we show efficient emission depletion for a class of lanthanide-doped upconversion nanoparticles with the assistance of interionic cross relaxation, which significantly lowers the laser intensity requirements of optical depletion. We demonstrate two-color super-resolution imaging using upconversion nanoparticles (resolution ~ 66 nm) with a single pair of excitation/depletion beams. In addition, we show super-resolution imaging of immunostained cytoskeleton structures of fixed cells (resolution ~ 82 nm) using upconversion nanoparticles. These achievements provide a new perspective for the development of photoswitchable luminescent probes and will broaden the applications of lanthanide-doped nanoparticles for sub-diffraction microscopic imaging.

Date: 2017
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DOI: 10.1038/s41467-017-01141-y

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