LRPPRC-mediated folding of the mitochondrial transcriptome

Stefan J. Siira, Henrik Spåhr, Anne-Marie J. Shearwood, Benedetta Ruzzenente, Nils-Göran Larsson, Oliver Rackham () and Aleksandra Filipovska ()
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Stefan J. Siira: The University of Western Australia
Henrik Spåhr: Max Planck Institute for Biology of Ageing
Anne-Marie J. Shearwood: The University of Western Australia
Benedetta Ruzzenente: Max Planck Institute for Biology of Ageing
Nils-Göran Larsson: Max Planck Institute for Biology of Ageing
Oliver Rackham: The University of Western Australia
Aleksandra Filipovska: The University of Western Australia

Nature Communications, 2017, vol. 8, issue 1, 1-11

Abstract: Abstract The expression of the compact mammalian mitochondrial genome requires transcription, RNA processing, translation and RNA decay, much like the more complex chromosomal systems, and here we use it as a model system to understand the fundamental aspects of gene expression. Here we combine RNase footprinting with PAR-CLIP at unprecedented depth to reveal the importance of RNA–protein interactions in dictating RNA folding within the mitochondrial transcriptome. We show that LRPPRC, in complex with its protein partner SLIRP, binds throughout the mitochondrial transcriptome, with a preference for mRNAs, and its loss affects the entire secondary structure and stability of the transcriptome. We demonstrate that the LRPPRC–SLIRP complex is a global RNA chaperone that stabilizes RNA structures to expose the required sites for translation, stabilization, and polyadenylation. Our findings reveal a general mechanism where extensive RNA–protein interactions ensure that RNA is accessible for its biological functions.

Date: 2017
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DOI: 10.1038/s41467-017-01221-z

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