Live imaging molecular changes in junctional tension upon VE-cadherin in zebrafish

Anne Karine Lagendijk (), Guillermo A. Gomez, Sungmin Baek, Daniel Hesselson, William E. Hughes, Scott Paterson, Daniel E. Conway, Heinz-Georg Belting, Markus Affolter, Kelly A. Smith, Martin A. Schwartz, Alpha S. Yap and Benjamin M. Hogan
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Anne Karine Lagendijk: Institute for Molecular Bioscience, Genomics of Development and Disease division, The University of Queensland
Guillermo A. Gomez: The University of Queensland
Sungmin Baek: Institute for Molecular Bioscience, Genomics of Development and Disease division, The University of Queensland
Daniel Hesselson: Garvan Institute of Medical Research
William E. Hughes: Garvan Institute of Medical Research
Scott Paterson: Institute for Molecular Bioscience, Genomics of Development and Disease division, The University of Queensland
Daniel E. Conway: Virginia Commonwealth University
Heinz-Georg Belting: Biozentrum der Universität Basel
Markus Affolter: Biozentrum der Universität Basel
Kelly A. Smith: Institute for Molecular Bioscience, Genomics of Development and Disease division, The University of Queensland
Martin A. Schwartz: Yale University School of Medicine
Alpha S. Yap: The University of Queensland
Benjamin M. Hogan: Institute for Molecular Bioscience, Genomics of Development and Disease division, The University of Queensland

Nature Communications, 2017, vol. 8, issue 1, 1-12

Abstract: Abstract Forces play diverse roles in vascular development, homeostasis and disease. VE-cadherin at endothelial cell-cell junctions links the contractile acto-myosin cytoskeletons of adjacent cells, serving as a tension-transducer. To explore tensile changes across VE-cadherin in live zebrafish, we tailored an optical biosensor approach, originally established in vitro. We validate localization and function of a VE-cadherin tension sensor (TS) in vivo. Changes in tension across VE-cadherin observed using ratio-metric or lifetime FRET measurements reflect acto-myosin contractility within endothelial cells. Furthermore, we apply the TS to reveal biologically relevant changes in VE-cadherin tension that occur as the dorsal aorta matures and upon genetic and chemical perturbations during embryonic development.

Date: 2017
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DOI: 10.1038/s41467-017-01325-6

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