DNA Ligase C and Prim-PolC participate in base excision repair in mycobacteria

Przemysław Płociński, Nigel C. Brissett, Julie Bianchi, Anna Brzostek, Małgorzata Korycka-Machała, Andrzej Dziembowski, Jarosław Dziadek and Aidan J. Doherty ()
Additional contact information
Przemysław Płociński: University of Sussex
Nigel C. Brissett: University of Sussex
Julie Bianchi: University of Sussex
Anna Brzostek: Polish Academy of Sciences
Małgorzata Korycka-Machała: Polish Academy of Sciences
Andrzej Dziembowski: Polish Academy of Sciences
Jarosław Dziadek: Polish Academy of Sciences
Aidan J. Doherty: University of Sussex

Nature Communications, 2017, vol. 8, issue 1, 1-12

Abstract: Abstract Prokaryotic Ligase D is a conserved DNA repair apparatus processing DNA double-strand breaks in stationary phase. An orthologous Ligase C (LigC) complex also co-exists in many bacterial species but its function is unknown. Here we show that the LigC complex interacts with core BER enzymes in vivo and demonstrate that together these factors constitute an excision repair apparatus capable of repairing damaged bases and abasic sites. The polymerase component, which contains a conserved C-terminal structural loop, preferentially binds to and fills-in short gapped DNA intermediates with RNA and LigC ligates the resulting nicks to complete repair. Components of the LigC complex, like LigD, are expressed upon entry into stationary phase and cells lacking either of these pathways exhibit increased sensitivity to oxidising genotoxins. Together, these findings establish that the LigC complex is directly involved in an excision repair pathway(s) that repairs DNA damage with ribonucleotides during stationary phase.

Date: 2017
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-017-01365-y Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-01365-y

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-017-01365-y

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().