Cytoplasmic cleavage of DPPA3 is required for intracellular trafficking and cleavage-stage development in mice

Seung-Wook Shin, Edgar John Vogt, Maria Jimenez-Movilla, Boris Baibakov and Jurrien Dean ()
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Seung-Wook Shin: NIDDK, National Institutes of Health
Edgar John Vogt: NIDDK, National Institutes of Health
Maria Jimenez-Movilla: Medical School, University of Murcia, IMIB
Boris Baibakov: NIDDK, National Institutes of Health
Jurrien Dean: NIDDK, National Institutes of Health

Nature Communications, 2017, vol. 8, issue 1, 1-12

Abstract: Abstract Degradation of maternal proteins by the ubiquitin-proteasome system (UPS) accompanies the maternal-to-zygotic transition. DPPA3/Stella/PGC7, encoded by a maternal effect gene, is present in the nucleus and cytoplasm of zygotes and has been associated with protecting the female pronucleus from TET3-mediated demethylation. We now report that cytoplasmic DPPA3 is partially cleaved by the ubiquitin-proteasome system and an N-terminus fragment remains in the cytoplasm where it associates with early and re-cycling endosomes. If DPPA3 is absent or if cleavage is prevented, multiple vesicles coalesce/aggregate and markers of lysosomes are decreased. Fertilized eggs develop poorly into blastocysts, which results in significantly decreased fecundity of Dppa3 R60A transgenic mice. This phenocopies aspects of Lamp1/2 knockdowns and Dppa3 KO embryos can be partially rescued in vitro by DPPA31–60 and to a lesser extent by LAMP1/2. Thus, the N-terminus of DPPA3 has a significant role in cytoplasmic vesicular trafficking in addition to its previously reported nuclear function.

Date: 2017
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DOI: 10.1038/s41467-017-01387-6

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