Contribution of epigenetic landscapes and transcription factors to X-chromosome reactivation in the inner cell mass

Maud Borensztein, Ikuhiro Okamoto, Laurène Syx, Guillaume Guilbaud, Christel Picard, Katia Ancelin, Rafael Galupa, Patricia Diabangouaya, Nicolas Servant, Emmanuel Barillot, Azim Surani, Mitinori Saitou, Chong-Jian Chen, Konstantinos Anastassiadis and Edith Heard ()
Additional contact information
Maud Borensztein: PSL Research University, CNRS UMR3215, INSERM U934, UPMC Paris-Sorbonne
Ikuhiro Okamoto: PSL Research University, CNRS UMR3215, INSERM U934, UPMC Paris-Sorbonne
Laurène Syx: PSL Research University, CNRS UMR3215, INSERM U934, UPMC Paris-Sorbonne
Guillaume Guilbaud: Medical Research Council Laboratory of Molecular Biology
Christel Picard: PSL Research University, CNRS UMR3215, INSERM U934, UPMC Paris-Sorbonne
Katia Ancelin: PSL Research University, CNRS UMR3215, INSERM U934, UPMC Paris-Sorbonne
Rafael Galupa: PSL Research University, CNRS UMR3215, INSERM U934, UPMC Paris-Sorbonne
Patricia Diabangouaya: PSL Research University, CNRS UMR3215, INSERM U934, UPMC Paris-Sorbonne
Nicolas Servant: PSL Research University, Mines Paris Tech, INSERM U900
Emmanuel Barillot: PSL Research University, Mines Paris Tech, INSERM U900
Azim Surani: University of Cambridge
Mitinori Saitou: Kyoto University
Chong-Jian Chen: Annoroad Gene Technology Co., Ltd
Konstantinos Anastassiadis: Technische Universität Dresden
Edith Heard: PSL Research University, CNRS UMR3215, INSERM U934, UPMC Paris-Sorbonne

Nature Communications, 2017, vol. 8, issue 1, 1-14

Abstract: Abstract X-chromosome inactivation is established during early development. In mice, transcriptional repression of the paternal X-chromosome (Xp) and enrichment in epigenetic marks such as H3K27me3 is achieved by the early blastocyst stage. X-chromosome inactivation is then reversed in the inner cell mass. The mechanisms underlying Xp reactivation remain enigmatic. Using in vivo single-cell approaches (allele-specific RNAseq, nascent RNA-fluorescent in situ hybridization and immunofluorescence), we show here that different genes are reactivated at different stages, with more slowly reactivated genes tending to be enriched in H3meK27. We further show that in UTX H3K27 histone demethylase mutant embryos, these genes are even more slowly reactivated, suggesting that these genes carry an epigenetic memory that may be actively lost. On the other hand, expression of rapidly reactivated genes may be driven by transcription factors. Thus, some X-linked genes have minimal epigenetic memory in the inner cell mass, whereas others may require active erasure of chromatin marks.

Date: 2017
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-017-01415-5 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-01415-5

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-017-01415-5

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().