Tsg101 chaperone function revealed by HIV-1 assembly inhibitors

Madeleine Strickland, Lorna S. Ehrlich, Susan Watanabe, Mahfuz Khan, Marie-Paule Strub, Chi-Hao Luan, Michael D. Powell, Jonathan Leis, Nico Tjandra () and Carol A. Carter ()
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Madeleine Strickland: Laboratory of Molecular Biophysics, Biochemistry and Biophysics Center, National Heart, Lung and Blood Institute, National Institutes of Health
Lorna S. Ehrlich: School of Medicine, Stony Brook University
Susan Watanabe: School of Medicine, Stony Brook University
Mahfuz Khan: Morehouse School of Medicine
Marie-Paule Strub: Laboratory of Molecular Biophysics, Biochemistry and Biophysics Center, National Heart, Lung and Blood Institute, National Institutes of Health
Chi-Hao Luan: Northwestern University
Michael D. Powell: Morehouse School of Medicine
Jonathan Leis: Feinberg School of Medicine, Northwestern University
Nico Tjandra: Laboratory of Molecular Biophysics, Biochemistry and Biophysics Center, National Heart, Lung and Blood Institute, National Institutes of Health
Carol A. Carter: School of Medicine, Stony Brook University

Nature Communications, 2017, vol. 8, issue 1, 1-12

Abstract: Abstract HIV-1 replication requires Tsg101, a component of cellular endosomal sorting complex required for transport (ESCRT) machinery. Tsg101 possesses an ubiquitin (Ub) E2 variant (UEV) domain with a pocket that can bind PT/SAP motifs and another pocket that can bind Ub. The PTAP motif in the viral structural precursor polyprotein, Gag, allows the recruitment of Tsg101 and other ESCRTs to virus assembly sites where they mediate budding. It is not known how or even whether the UEV Ub binding function contributes to virus production. Here, we report that disruption of UEV Ub binding by commonly used drugs arrests assembly at an early step distinct from the late stage involving PTAP binding disruption. NMR reveals that the drugs form a covalent adduct near the Ub-binding pocket leading to the disruption of Ub, but not PTAP binding. We conclude that the Ub-binding pocket has a chaperone function involved in bud initiation.

Date: 2017
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DOI: 10.1038/s41467-017-01426-2

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