Longitudinal intravital imaging of the femoral bone marrow reveals plasticity within marrow vasculature

David Reismann, Jonathan Stefanowski, Robert Günther, Asylkhan Rakhymzhan, Romano Matthys, Reto Nützi, Sandra Zehentmeier, Katharina Schmidt-Bleek, Georg Petkau, Hyun-Dong Chang, Sandra Naundorf, York Winter, Fritz Melchers, Georg Duda, Anja E. Hauser () and Raluca A. Niesner ()
Additional contact information
David Reismann: A Leibniz Institute, Charitéplatz 1
Jonathan Stefanowski: A Leibniz Institute, Charitéplatz 1
Robert Günther: A Leibniz Institute, Charitéplatz 1
Asylkhan Rakhymzhan: A Leibniz Institute, Charitéplatz 1
Romano Matthys: Talstraße 2A
Reto Nützi: Talstraße 2A
Sandra Zehentmeier: A Leibniz Institute, Charitéplatz 1
Katharina Schmidt-Bleek: Charité—Universitätsmedizin, Augustenburger Platz 1
Georg Petkau: A Leibniz Institute, Charitéplatz 1
Hyun-Dong Chang: A Leibniz Institute, Charitéplatz 1
Sandra Naundorf: A Leibniz Institute, Charitéplatz 1
York Winter: Unter den Linden 6
Fritz Melchers: A Leibniz Institute, Charitéplatz 1
Georg Duda: Charité—Universitätsmedizin, Augustenburger Platz 1
Anja E. Hauser: A Leibniz Institute, Charitéplatz 1
Raluca A. Niesner: A Leibniz Institute, Charitéplatz 1

Nature Communications, 2017, vol. 8, issue 1, 1-15

Abstract: Abstract The bone marrow is a central organ of the immune system, which hosts complex interactions of bone and immune compartments critical for hematopoiesis, immunological memory, and bone regeneration. Although these processes take place over months, most existing imaging techniques allow us to follow snapshots of only a few hours, at subcellular resolution. Here, we develop a microendoscopic multi-photon imaging approach called LIMB (longitudinal intravital imaging of the bone marrow) to analyze cellular dynamics within the deep marrow. The approach consists of a biocompatible plate surgically fixated to the mouse femur containing a gradient refractive index lens. This microendoscope allows highly resolved imaging, repeatedly at the same regions within marrow tissue, over months. LIMB reveals extensive vascular plasticity during bone healing and steady-state homeostasis. To our knowledge, this vascular plasticity is unique among mammalian tissues, and we expect this insight will decisively change our understanding of essential phenomena occurring within the bone marrow.

Date: 2017
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DOI: 10.1038/s41467-017-01538-9

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