Antagonistic bacteria disrupt calcium homeostasis and immobilize algal cells

Prasad Aiyar, Daniel Schaeme, María García-Altares, David Carrasco Flores, Hannes Dathe, Christian Hertweck, Severin Sasso () and Maria Mittag ()
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Prasad Aiyar: Friedrich Schiller University, Am Planetarium 1
Daniel Schaeme: Friedrich Schiller University, Am Planetarium 1
María García-Altares: Beutenbergstr. 11 a
David Carrasco Flores: Friedrich Schiller University, Am Planetarium 1
Hannes Dathe: Friedrich Schiller University, Am Planetarium 1
Christian Hertweck: Beutenbergstr. 11 a
Severin Sasso: Friedrich Schiller University, Am Planetarium 1
Maria Mittag: Friedrich Schiller University, Am Planetarium 1

Nature Communications, 2017, vol. 8, issue 1, 1-13

Abstract: Abstract Photosynthetic unicellular organisms, known as microalgae, are key contributors to carbon fixation on Earth. Their biotic interactions with other microbes shape aquatic microbial communities and influence the global photosynthetic capacity. So far, limited information is available on molecular factors that govern these interactions. We show that the bacterium Pseudomonas protegens strongly inhibits the growth and alters the morphology of the biflagellated green alga Chlamydomonas reinhardtii. This antagonistic effect is decreased in a bacterial mutant lacking orfamides, demonstrating that these secreted cyclic lipopeptides play an important role in the algal–bacterial interaction. Using an aequorin Ca2+-reporter assay, we show that orfamide A triggers an increase in cytosolic Ca2+ in C. reinhardtii and causes deflagellation of algal cells. These effects of orfamide A, which are specific to the algal class of Chlorophyceae and appear to target a Ca2+ channel in the plasma membrane, represent a novel biological activity for cyclic lipopeptides.

Date: 2017
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DOI: 10.1038/s41467-017-01547-8

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