Influenza virus genome reaches the plasma membrane via a modified endoplasmic reticulum and Rab11-dependent vesicles

Isabel Fernández de Castro Martin, Guillaume Fournier, Martin Sachse, Javier Pizarro-Cerda, Cristina Risco () and Nadia Naffakh ()
Additional contact information
Isabel Fernández de Castro Martin: Centro Nacional de Biotecnologia, CNB-CSIC, Department of Macromolecular Structures, Cell Structure Lab
Guillaume Fournier: Institut Pasteur, Unité de Génétique Moléculaire des Virus à ARN
Martin Sachse: Center for Innovation and Technological Research, Ultrastructural Bio-imaging, Institut Pasteur
Javier Pizarro-Cerda: Institut Pasteur, Unité des Interactions Bactéries-Cellules
Cristina Risco: Centro Nacional de Biotecnologia, CNB-CSIC, Department of Macromolecular Structures, Cell Structure Lab
Nadia Naffakh: Institut Pasteur, Unité de Génétique Moléculaire des Virus à ARN

Nature Communications, 2017, vol. 8, issue 1, 1-12

Abstract: Abstract Transport of neo-synthesized influenza A virus (IAV) viral ribonucleoproteins (vRNPs) from the nucleus to the plasma membrane involves Rab 11 but the precise mechanism remains poorly understood. We used metal-tagging and immunolabeling to visualize viral proteins and cellular endomembrane markers by electron microscopy of IAV-infected cells. Unexpectedly, we provide evidence that the vRNP components and the Rab11 protein are present at the membrane of a modified, tubulated endoplasmic reticulum (ER) that extends all throughout the cell, and on irregularly coated vesicles (ICVs). Some ICVs are found very close to the ER and to the plasma membrane. ICV formation is observed only in infected cells and requires an active Rab11 GTPase. Against the currently accepted model in which vRNPs are carried onto Rab11-positive recycling endosomes across the cytoplasm, our findings reveal that the endomembrane organelle that is primarily involved in the transport of vRNPs is the ER.

Date: 2017
References: Add references at CitEc
Citations: View citations in EconPapers (1)

Downloads: (external link)
https://www.nature.com/articles/s41467-017-01557-6 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-01557-6

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-017-01557-6

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().