UNC93B1 interacts with the calcium sensor STIM1 for efficient antigen cross-presentation in dendritic cells

Sophia Maschalidi, Paula Nunes-Hasler, Clarissa R Nascimento, Ignacio Sallent, Valérie Lannoy, Meriem Garfa-Traore, Nicolas Cagnard, Fernando E. Sepulveda, Pablo Vargas, Ana-Maria Lennon-Duménil, Peter Endert, Thierry Capiod, Nicolas Demaurex, Guillaume Darrasse-Jèze and Bénédicte Manoury ()
Additional contact information
Sophia Maschalidi: INSERM UMR1163, Laboratory of Normal and Pathological Homeostasis of the Immune System, Imagine Institute
Paula Nunes-Hasler: University of Geneva
Clarissa R Nascimento: Université Paris Descartes
Ignacio Sallent: Université Paris Descartes
Valérie Lannoy: Université Paris Descartes
Meriem Garfa-Traore: Université Paris Descartes
Nicolas Cagnard: Université Paris Descartes
Fernando E. Sepulveda: INSERM UMR1163, Laboratory of Normal and Pathological Homeostasis of the Immune System, Imagine Institute
Pablo Vargas: Institut Curie, PSL Research University, Centre National de la Recherche Scientifique, UMR 144
Ana-Maria Lennon-Duménil: Institut National de la Santé et de la Recherché Médicale, Unité 932, Institut Curie, PSL Research University
Peter Endert: Université Paris Descartes
Thierry Capiod: Université Paris Descartes
Nicolas Demaurex: University of Geneva
Guillaume Darrasse-Jèze: Université Paris Descartes
Bénédicte Manoury: Université Paris Descartes

Nature Communications, 2017, vol. 8, issue 1, 1-16

Abstract: Abstract Dendritic cells (DC) have the unique ability to present exogenous antigens via the major histocompatibility complex class I pathway to stimulate naive CD8+ T cells. In DCs with a non-functional mutation in Unc93b1 (3d mutation), endosomal acidification, phagosomal maturation, antigen degradation, antigen export to the cytosol and the function of the store-operated-Ca2+-entry regulator STIM1 are impaired. These defects result in compromised antigen cross-presentation and anti-tumor responses in 3d-mutated mice. Here, we show that UNC93B1 interacts with the calcium sensor STIM1 in the endoplasmic reticulum, a critical step for STIM1 oligomerization and activation. Expression of a constitutively active STIM1 mutant, which no longer binds UNC93B1, restores antigen degradation and cross-presentation in 3d-mutated DCs. Furthermore, ablation of STIM1 in mouse and human cells leads to a decrease in cross-presentation. Our data indicate that the UNC93B1 and STIM1 cooperation is important for calcium flux and antigen cross-presentation in DCs.

Date: 2017
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DOI: 10.1038/s41467-017-01601-5

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