Novel genetically encoded fluorescent probes enable real-time detection of potassium in vitro and in vivo

Helmut Bischof, Markus Rehberg, Sarah Stryeck, Katharina Artinger, Emrah Eroglu, Markus Waldeck-Weiermair, Benjamin Gottschalk, Rene Rost, Andras T. Deak, Tobias Niedrist, Nemanja Vujic, Hanna Lindermuth, Ruth Prassl, Brigitte Pelzmann, Klaus Groschner, Dagmar Kratky, Kathrin Eller, Alexander R. Rosenkranz, Tobias Madl, Nikolaus Plesnila, Wolfgang F. Graier and Roland Malli ()
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Helmut Bischof: Medical University of Graz
Markus Rehberg: Klinikum der Universität München
Sarah Stryeck: Medical University of Graz
Katharina Artinger: Medical University of Graz
Emrah Eroglu: Medical University of Graz
Markus Waldeck-Weiermair: Medical University of Graz
Benjamin Gottschalk: Medical University of Graz
Rene Rost: Medical University of Graz
Andras T. Deak: Medical University of Graz
Tobias Niedrist: Medical University of Graz
Nemanja Vujic: Medical University of Graz
Hanna Lindermuth: Medical University of Graz
Ruth Prassl: Medical University of Graz
Brigitte Pelzmann: Medical University of Graz
Klaus Groschner: Medical University of Graz
Dagmar Kratky: Medical University of Graz
Kathrin Eller: Medical University of Graz
Alexander R. Rosenkranz: Medical University of Graz
Tobias Madl: Medical University of Graz
Nikolaus Plesnila: Klinikum der Universität München
Wolfgang F. Graier: Medical University of Graz
Roland Malli: Medical University of Graz

Nature Communications, 2017, vol. 8, issue 1, 1-12

Abstract: Abstract Changes in intra- and extracellular potassium ion (K+) concentrations control many important cellular processes and related biological functions. However, our current understanding of the spatiotemporal patterns of physiological and pathological K+ changes is severely limited by the lack of practicable detection methods. We developed K+-sensitive genetically encoded, Förster resonance energy transfer-(FRET) based probes, called GEPIIs, which enable quantitative real-time imaging of K+ dynamics. GEPIIs as purified biosensors are suitable to directly and precisely quantify K+ levels in different body fluids and cell growth media. GEPIIs expressed in cells enable time-lapse and real-time recordings of global and local intracellular K+ signals. Hitherto unknown Ca2+-triggered, organelle-specific K+ changes were detected in pancreatic beta cells. Recombinant GEPIIs also enabled visualization of extracellular K+ fluctuations in vivo with 2-photon microscopy. Therefore, GEPIIs are relevant for diverse K+ assays and open new avenues for live-cell K+ imaging.

Date: 2017
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DOI: 10.1038/s41467-017-01615-z

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