Label-free automated three-dimensional imaging of whole organs by microtomy-assisted photoacoustic microscopy

Terence T. W. Wong, Ruiying Zhang, Chi Zhang, Hsun-Chia Hsu, Konstantin I. Maslov, Lidai Wang, Junhui Shi, Ruimin Chen, K. Kirk Shung, Qifa Zhou and Lihong V. Wang ()
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Terence T. W. Wong: California Institute of Technology
Ruiying Zhang: Washington University in St. Louis
Chi Zhang: Washington University in St. Louis
Hsun-Chia Hsu: California Institute of Technology
Konstantin I. Maslov: California Institute of Technology
Lidai Wang: Washington University in St. Louis
Junhui Shi: California Institute of Technology
Ruimin Chen: University of Southern California
K. Kirk Shung: University of Southern California
Qifa Zhou: University of Southern California
Lihong V. Wang: California Institute of Technology

Nature Communications, 2017, vol. 8, issue 1, 1-8

Abstract: Abstract Three-dimensional (3D) optical imaging of whole biological organs with microscopic resolution has remained a challenge. Most versions of such imaging techniques require special preparation of the tissue specimen. Here we demonstrate microtomy-assisted photoacoustic microscopy (mPAM) of mouse brains and other organs, which automatically acquires serial distortion-free and registration-free images with endogenous absorption contrasts. Without tissue staining or clearing, mPAM generates micrometer-resolution 3D images of paraffin- or agarose-embedded whole organs with high fidelity, achieved by label-free simultaneous sensing of DNA/RNA, hemoglobins, and lipids. mPAM provides histology-like imaging of cell nuclei, blood vessels, axons, and other anatomical structures, enabling the application of histopathological interpretation at the organelle level to analyze a whole organ. Its deep tissue imaging capability leads to less sectioning, resulting in negligible sectioning artifact. mPAM offers a new way to better understand complex biological organs.

Date: 2017
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DOI: 10.1038/s41467-017-01649-3

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