Fusion guide RNAs for orthogonal gene manipulation with Cas9 and Cpf1

Jiyeon Kweon, An-Hee Jang, Da-eun Kim, Jin Wook Yang, Mijung Yoon, Ha Rim Shin, Jin-Soo Kim and Yongsub Kim ()
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Jiyeon Kweon: University of Ulsan College of Medicine, Asan Medical Center
An-Hee Jang: University of Ulsan College of Medicine, Asan Medical Center
Da-eun Kim: Seoul National University
Jin Wook Yang: University of Ulsan College of Medicine, Asan Medical Center
Mijung Yoon: University of Ulsan College of Medicine, Asan Medical Center
Ha Rim Shin: University of Ulsan College of Medicine, Asan Medical Center
Jin-Soo Kim: Seoul National University
Yongsub Kim: University of Ulsan College of Medicine, Asan Medical Center

Nature Communications, 2017, vol. 8, issue 1, 1-6

Abstract: Abstract The bacteria-derived clustered regularly interspaced short palindromic repeat (CRISPR)–Cas systems are powerful tools for genome engineering. Recently, in addition to Cas protein engineering, the improvement of guide RNAs are also performed, contributing to broadening the research area of CRISPR–Cas9 systems. Here we develop a fusion guide RNA (fgRNA) that functions with both Cas9 and Cpf1 proteins to induce mutations in human cells. Furthermore, we demonstrate that fgRNAs can be used in multiplex genome editing and orthogonal genome manipulation with two types of Cas proteins. Our results show that fgRNAs can be used as a tool for performing multiple gene manipulations.

Date: 2017
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DOI: 10.1038/s41467-017-01650-w

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