Regulation of gene expression and RNA editing in Drosophila adapting to divergent microclimates

Arielle L. Yablonovitch, Jeremy Fu, Kexin Li, Simpla Mahato, Lin Kang, Eugenia Rashkovetsky, Abraham B. Korol, Hua Tang, Pawel Michalak, Andrew C. Zelhof, Eviatar Nevo () and Jin Billy Li ()
Additional contact information
Arielle L. Yablonovitch: Stanford University School of Medicine
Jeremy Fu: Stanford University School of Medicine
Kexin Li: University of Haifa
Simpla Mahato: Indiana University
Lin Kang: Edward Via College of Osteopathic Medicine
Eugenia Rashkovetsky: University of Haifa
Abraham B. Korol: University of Haifa
Hua Tang: Stanford University School of Medicine
Pawel Michalak: Edward Via College of Osteopathic Medicine
Andrew C. Zelhof: Indiana University
Eviatar Nevo: University of Haifa
Jin Billy Li: Stanford University School of Medicine

Nature Communications, 2017, vol. 8, issue 1, 1-14

Abstract: Abstract Determining the mechanisms by which a species adapts to its environment is a key endeavor in the study of evolution. In particular, relatively little is known about how transcriptional processes are fine-tuned to adjust to different environmental conditions. Here we study Drosophila melanogaster from ‘Evolution Canyon’ in Israel, which consists of two opposing slopes with divergent microclimates. We identify several hundred differentially expressed genes and dozens of differentially edited sites between flies from each slope, correlate these changes with genetic differences, and use CRISPR mutagenesis to validate that an intronic SNP in prominin regulates its editing levels. We also demonstrate that while temperature affects editing levels at more sites than genetic differences, genetically regulated sites tend to be less affected by temperature. This work shows the extent to which gene expression and RNA editing differ between flies from different microclimates, and provides insights into the regulation responsible for these differences.

Date: 2017
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-017-01658-2 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-01658-2

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-017-01658-2

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().