Semisynthetic fluorescent pH sensors for imaging exocytosis and endocytosis

Magalie Martineau, Agila Somasundaram, Jonathan B. Grimm, Todd D. Gruber, Daniel Choquet, Justin W. Taraska (), Luke D. Lavis () and David Perrais ()
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Magalie Martineau: University of Bordeaux
Agila Somasundaram: US National Institutes of Health
Jonathan B. Grimm: Howard Hughes Medical Institute
Todd D. Gruber: Howard Hughes Medical Institute
Daniel Choquet: University of Bordeaux
Justin W. Taraska: US National Institutes of Health
Luke D. Lavis: Howard Hughes Medical Institute
David Perrais: University of Bordeaux

Nature Communications, 2017, vol. 8, issue 1, 1-10

Abstract: Abstract The GFP-based superecliptic pHluorin (SEP) enables detection of exocytosis and endocytosis, but its performance has not been duplicated in red fluorescent protein scaffolds. Here we describe “semisynthetic” pH-sensitive protein conjugates with organic fluorophores, carbofluorescein, and Virginia Orange that match the properties of SEP. Conjugation to genetically encoded self-labeling tags or antibodies allows visualization of both exocytosis and endocytosis, constituting new bright sensors for these key steps of synaptic transmission.

Date: 2017
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-01752-5

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DOI: 10.1038/s41467-017-01752-5

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