Structural basis for the assembly of the Ragulator-Rag GTPase complex

Ryo Yonehara, Shigeyuki Nada, Tomokazu Nakai, Masahiro Nakai, Ayaka Kitamura, Akira Ogawa, Hirokazu Nakatsumi, Keiichi I. Nakayama, Songling Li, Daron M. Standley, Eiki Yamashita, Atsushi Nakagawa () and Masato Okada ()
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Ryo Yonehara: Osaka University
Shigeyuki Nada: Osaka University
Tomokazu Nakai: Osaka University
Masahiro Nakai: Osaka University
Ayaka Kitamura: Osaka University
Akira Ogawa: Osaka University
Hirokazu Nakatsumi: Kyushu University
Keiichi I. Nakayama: Kyushu University
Songling Li: Osaka University
Daron M. Standley: Osaka University
Eiki Yamashita: Osaka University
Atsushi Nakagawa: Osaka University
Masato Okada: Osaka University

Nature Communications, 2017, vol. 8, issue 1, 1-11

Abstract: Abstract The mechanistic target of rapamycin complex 1 (mTORC1) plays a central role in regulating cell growth and metabolism by responding to cellular nutrient conditions. The activity of mTORC1 is controlled by Rag GTPases, which are anchored to lysosomes via Ragulator, a pentameric protein complex consisting of membrane-anchored p18/LAMTOR1 and two roadblock heterodimers. Here we report the crystal structure of Ragulator in complex with the roadblock domains of RagA-C, which helps to elucidate the molecular basis for the regulation of Rag GTPases. In the structure, p18 wraps around the three pairs of roadblock heterodimers to tandemly assemble them onto lysosomes. Cellular and in vitro analyses further demonstrate that p18 is required for Ragulator-Rag GTPase assembly and amino acid-dependent activation of mTORC1. These results establish p18 as a critical organizing scaffold for the Ragulator-Rag GTPase complex, which may provide a platform for nutrient sensing on lysosomes.

Date: 2017
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DOI: 10.1038/s41467-017-01762-3

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