Quantitative analysis of protein-protein interactions and post-translational modifications in rare immune populations

Ayelet Avin, Maayan Levy, Ziv Porat and Jakub Abramson ()
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Ayelet Avin: Weizmann Institute of Science
Maayan Levy: Weizmann Institute of Science
Ziv Porat: Weizmann Institute of Science
Jakub Abramson: Weizmann Institute of Science

Nature Communications, 2017, vol. 8, issue 1, 1-10

Abstract: Abstract In spite of recent advances in proteomics, quantitative analyses of protein–protein interactions (PPIs) or post-translational modifications (PTMs) in rare cell populations remain challenging. This is in particular true for analyses of rare immune and/or stem cell populations that are directly isolated from humans or animal models, and which are often characterized by multiple surface markers. To overcome these limitations, here we have developed proximity ligation imaging cytometry (PLIC), a protocol for proteomic analysis of rare cells. Specifically, by employing PLIC on medullary thymic epithelial cells (mTECs), which serve as a paradigm for a rare immune population, we demonstrate that PLIC overcomes the inherent limitations of conventional proteomic approaches and enables a high-resolution detection and quantification of PPIs and PTMs at a single cell level.

Date: 2017
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DOI: 10.1038/s41467-017-01808-6

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