In situ detection of the protein corona in complex environments

Monica Carril (), Daniel Padro, Pablo del Pino, Carolina Carrillo-Carrion, Marta Gallego and Wolfgang J. Parak ()
Additional contact information
Monica Carril: CIC biomaGUNE
Daniel Padro: CIC biomaGUNE
Pablo del Pino: CIC biomaGUNE
Carolina Carrillo-Carrion: CIC biomaGUNE
Marta Gallego: CIC biomaGUNE
Wolfgang J. Parak: CIC biomaGUNE

Nature Communications, 2017, vol. 8, issue 1, 1-5

Abstract: Abstract Colloidal nanoparticles (NPs) are a versatile potential platform for in vivo nanomedicine. Inside blood circulation, NPs may undergo drastic changes, such as by formation of a protein corona. The in vivo corona cannot be completely emulated by the corona formed in blood. Thus, in situ detection in complex media, and ultimately in vivo, is required. Here we present a methodology for determining protein corona formation in complex media. NPs are labeled with 19F and their diffusion coefficient measured using 19F diffusion-ordered nuclear magnetic resonance (NMR) spectroscopy. 19F diffusion NMR measurements of hydrodynamic radii allow for in situ characterization of NPs in complex environments by quantification of protein adsorption to the surface of NPs, as determined by increase in hydrodynamic radius. The methodology is not optics based, and thus can be used in turbid environments, as in the presence of cells.

Date: 2017
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DOI: 10.1038/s41467-017-01826-4

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