CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing

Miguel A. Moreno-Mateos (), Juan P. Fernandez, Romain Rouet, Charles E. Vejnar, Maura A. Lane, Emily Mis, Mustafa K. Khokha, Jennifer A. Doudna and Antonio J. Giraldez ()
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Miguel A. Moreno-Mateos: Yale University School of Medicine
Juan P. Fernandez: Yale University School of Medicine
Romain Rouet: University of California
Charles E. Vejnar: Yale University School of Medicine
Maura A. Lane: Yale University School of Medicine
Emily Mis: Yale University School of Medicine
Mustafa K. Khokha: Yale University School of Medicine
Jennifer A. Doudna: University of California
Antonio J. Giraldez: Yale University School of Medicine

Nature Communications, 2017, vol. 8, issue 1, 1-9

Abstract: Abstract Cpf1 is a novel class of CRISPR-Cas DNA endonucleases, with a wide range of activity across different eukaryotic systems. Yet, the underlying determinants of this variability are poorly understood. Here, we demonstrate that LbCpf1, but not AsCpf1, ribonucleoprotein complexes allow efficient mutagenesis in zebrafish and Xenopus. We show that temperature modulates Cpf1 activity by controlling its ability to access genomic DNA. This effect is stronger on AsCpf1, explaining its lower efficiency in ectothermic organisms. We capitalize on this property to show that temporal control of the temperature allows post-translational modulation of Cpf1-mediated genome editing. Finally, we determine that LbCpf1 significantly increases homology-directed repair in zebrafish, improving current approaches for targeted DNA integration in the genome. Together, we provide a molecular understanding of Cpf1 activity in vivo and establish Cpf1 as an efficient and inducible genome engineering tool across ectothermic species.

Date: 2017
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DOI: 10.1038/s41467-017-01836-2

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