Cryo-EM structure of Saccharomyces cerevisiae target of rapamycin complex 2

Manikandan Karuppasamy, Beata Kusmider, Taiana M. Oliveira, Christl Gaubitz, Manoel Prouteau, Robbie Loewith () and Christiane Schaffitzel ()
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Manikandan Karuppasamy: Grenoble Outstation
Beata Kusmider: University of Geneva
Taiana M. Oliveira: Grenoble Outstation
Christl Gaubitz: University of Geneva
Manoel Prouteau: University of Geneva
Robbie Loewith: University of Geneva
Christiane Schaffitzel: Grenoble Outstation

Nature Communications, 2017, vol. 8, issue 1, 1-10

Abstract: Abstract The target of rapamycin (TOR) kinase assembles into two distinct multiprotein complexes, conserved across eukaryote evolution. In contrast to TOR complex 1 (TORC1), TORC2 kinase activity is not inhibited by the macrolide rapamycin. Here, we present the structure of Saccharomyces cerevisiae TORC2 determined by electron cryo-microscopy. TORC2 contains six subunits assembling into a 1.4 MDa rhombohedron. Tor2 and Lst8 form the common core of both TOR complexes. Avo3/Rictor is unique to TORC2, but interacts with the same HEAT repeats of Tor2 that are engaged by Kog1/Raptor in mammalian TORC1, explaining the mutual exclusivity of these two proteins. Density, which we conclude is Avo3, occludes the FKBP12-rapamycin-binding site of Tor2’s FRB domain rendering TORC2 rapamycin insensitive and recessing the kinase active site. Although mobile, Avo1/hSin1 further restricts access to the active site as its conserved-region-in-the-middle (CRIM) domain is positioned along an edge of the TORC2 active-site-cleft, consistent with a role for CRIM in substrate recruitment.

Date: 2017
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DOI: 10.1038/s41467-017-01862-0

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