Label-free nanoscale optical metrology on myelinated axons in vivo

Junhwan Kwon, Moonseok Kim, Hyejin Park, Bok-Man Kang, Yongjae Jo, Jae-Hwan Kim, Oliver James, Seok-Hyun Yun, Seong-Gi Kim, Minah Suh and Myunghwan Choi ()
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Junhwan Kwon: Institute for Basic Science (IBS)
Moonseok Kim: Harvard Medical School
Hyejin Park: Institute for Basic Science (IBS)
Bok-Man Kang: Institute for Basic Science (IBS)
Yongjae Jo: Institute for Basic Science (IBS)
Jae-Hwan Kim: Institute for Basic Science (IBS)
Oliver James: Institute for Basic Science (IBS)
Seok-Hyun Yun: Harvard Medical School
Seong-Gi Kim: Institute for Basic Science (IBS)
Minah Suh: Institute for Basic Science (IBS)
Myunghwan Choi: Institute for Basic Science (IBS)

Nature Communications, 2017, vol. 8, issue 1, 1-9

Abstract: Abstract In the mammalian nervous system, myelin provides electrical insulation for the neural circuit by forming a highly organized, multilayered thin film around the axon fibers. Here, we investigate the spectral reflectance from this subcellular nanostructure and devise a new label-free technique based on a spectroscopic analysis of reflected light, enabling nanoscale imaging of myelinated axons in their natural living state. Using this technique, we demonstrate three-dimensional mapping of the axon diameter and sensing of dynamic changes in the substructure of myelin at nanoscale. We further reveal the prevalence of axon bulging in the brain cortex in vivo after mild compressive trauma. Our novel tool opens new avenues of investigation by creating unprecedented access to the nanostructural dynamics of live myelinated axons in health and disease.

Date: 2017
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DOI: 10.1038/s41467-017-01979-2

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