Microbial volatile communication in human organotypic lung models

Layla J. Barkal, Clare L. Procknow, Yasmín R. Álvarez-García, Mengyao Niu, José A. Jiménez-Torres, Rebecca A. Brockman-Schneider, James E. Gern, Loren C. Denlinger, Ashleigh B. Theberge, Nancy P. Keller, Erwin Berthier () and David J. Beebe ()
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Layla J. Barkal: University of Wisconsin-Madison
Clare L. Procknow: University of Wisconsin-Madison
Yasmín R. Álvarez-García: University of Wisconsin-Madison
Mengyao Niu: University of Wisconsin-Madison
José A. Jiménez-Torres: University of Wisconsin-Madison
Rebecca A. Brockman-Schneider: University of Wisconsin-Madison
James E. Gern: University of Wisconsin-Madison
Loren C. Denlinger: University of Wisconsin-Madison
Ashleigh B. Theberge: University of Washington
Nancy P. Keller: University of Wisconsin-Madison
Erwin Berthier: University of Washington
David J. Beebe: University of Wisconsin-Madison

Nature Communications, 2017, vol. 8, issue 1, 1-10

Abstract: Abstract We inhale respiratory pathogens continuously, and the subsequent signaling events between host and microbe are complex, ultimately resulting in clearance of the microbe, stable colonization of the host, or active disease. Traditional in vitro methods are ill-equipped to study these critical events in the context of the lung microenvironment. Here we introduce a microscale organotypic model of the human bronchiole for studying pulmonary infection. By leveraging microscale techniques, the model is designed to approximate the structure of the human bronchiole, containing airway, vascular, and extracellular matrix compartments. To complement direct infection of the organotypic bronchiole, we present a clickable extension that facilitates volatile compound communication between microbial populations and the host model. Using Aspergillus fumigatus, a respiratory pathogen, we characterize the inflammatory response of the organotypic bronchiole to infection. Finally, we demonstrate multikingdom, volatile-mediated communication between the organotypic bronchiole and cultures of Aspergillus fumigatus and Pseudomonas aeruginosa.

Date: 2017
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-01985-4

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DOI: 10.1038/s41467-017-01985-4

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