Efficient transgenesis and annotated genome sequence of the regenerative flatworm model Macrostomum lignano

Jakub Wudarski, Daniil Simanov, Kirill Ustyantsev, Katrien Mulder, Margriet Grelling, Magda Grudniewska, Frank Beltman, Lisa Glazenburg, Turan Demircan, Julia Wunderer, Weihong Qi, Dita B. Vizoso, Philipp M. Weissert, Daniel Olivieri, Stijn Mouton, Victor Guryev, Aziz Aboobaker, Lukas Schärer, Peter Ladurner and Eugene Berezikov ()
Additional contact information
Jakub Wudarski: University of Groningen, University Medical Center Groningen, Antonius Deusinglaan 1
Daniil Simanov: University of Groningen, University Medical Center Groningen, Antonius Deusinglaan 1
Kirill Ustyantsev: Prospekt Lavrentyeva 10
Katrien Mulder: Uppsalalaan 8
Margriet Grelling: University of Groningen, University Medical Center Groningen, Antonius Deusinglaan 1
Magda Grudniewska: University of Groningen, University Medical Center Groningen, Antonius Deusinglaan 1
Frank Beltman: University of Groningen, University Medical Center Groningen, Antonius Deusinglaan 1
Lisa Glazenburg: University of Groningen, University Medical Center Groningen, Antonius Deusinglaan 1
Turan Demircan: Uppsalalaan 8
Julia Wunderer: University of Innsbruck, Technikerstr. 25
Weihong Qi: Winterthurerstrasse 190
Dita B. Vizoso: University of Basel, Vesalgasse 1
Philipp M. Weissert: University of Groningen, University Medical Center Groningen, Antonius Deusinglaan 1
Daniel Olivieri: University of Groningen, University Medical Center Groningen, Antonius Deusinglaan 1
Stijn Mouton: University of Groningen, University Medical Center Groningen, Antonius Deusinglaan 1
Victor Guryev: University of Groningen, University Medical Center Groningen, Antonius Deusinglaan 1
Aziz Aboobaker: University of Oxford, Tinbergen Building, South Parks Road
Lukas Schärer: University of Basel, Vesalgasse 1
Peter Ladurner: University of Innsbruck, Technikerstr. 25
Eugene Berezikov: University of Groningen, University Medical Center Groningen, Antonius Deusinglaan 1

Nature Communications, 2017, vol. 8, issue 1, 1-12

Abstract: Abstract Regeneration-capable flatworms are informative research models to study the mechanisms of stem cell regulation, regeneration, and tissue patterning. However, the lack of transgenesis methods considerably hampers their wider use. Here we report development of a transgenesis method for Macrostomum lignano, a basal flatworm with excellent regeneration capacity. We demonstrate that microinjection of DNA constructs into fertilized one-cell stage eggs, followed by a low dose of irradiation, frequently results in random integration of the transgene in the genome and its stable transmission through the germline. To facilitate selection of promoter regions for transgenic reporters, we assembled and annotated the M. lignano genome, including genome-wide mapping of transcription start regions, and show its utility by generating multiple stable transgenic lines expressing fluorescent proteins under several tissue-specific promoters. The reported transgenesis method and annotated genome sequence will permit sophisticated genetic studies on stem cells and regeneration using M. lignano as a model organism.

Date: 2017
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-02214-8

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DOI: 10.1038/s41467-017-02214-8

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