Atmospheric pressure mass spectrometric imaging of live hippocampal tissue slices with subcellular spatial resolution

Jae Young Kim, Eun Seok Seo, Hyunmin Kim, Ji-Won Park, Dong-Kwon Lim and Dae Won Moon ()
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Jae Young Kim: DGIST
Eun Seok Seo: DGIST
Hyunmin Kim: DGIST
Ji-Won Park: Chungnam National University
Dong-Kwon Lim: Korea University
Dae Won Moon: DGIST

Nature Communications, 2017, vol. 8, issue 1, 1-13

Abstract: Abstract We report a high spatial resolution mass spectrometry (MS) system that allows us to image live hippocampal tissue slices under open-air atmospheric pressure (AP) and ambient temperature conditions at the subcellular level. The method is based on an efficient desorption process by femtosecond (fs) laser assisted with nanoparticles and a subsequent ionization step by applying nonthermal plasma, termed AP nanoparticle and plasma assisted laser desorption ionization (AP-nanoPALDI) MS method. Combining the AP-nanoPALDI with microscopic sample scanning, MS imaging with spatial resolution of 2.9 µm was obtained. The observed AP-nanoPALDI MS imaging clearly revealed the differences of molecular composition between the apical and basal dendrite regions of a hippocampal tissue. In addition, the AP-nanoPALDI MS imaging showed the decrease of cholesterol in hippocampus by treating with methyl β-cyclodextrin, which exemplifies the potential of AP-nanoPALDI for live tissue imaging for various biomedical applications without any chemical pretreatment and/or labeling process.

Date: 2017
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DOI: 10.1038/s41467-017-02216-6

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