Structural insight into catalytic mechanism of PET hydrolase

Xu Han, Weidong Liu, Jian-Wen Huang, Jiantao Ma, Yingying Zheng, Tzu-Ping Ko, Limin Xu, Ya-Shan Cheng, Chun-Chi Chen and Rey-Ting Guo ()
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Xu Han: Chinese Academy of Sciences
Weidong Liu: Chinese Academy of Sciences
Jian-Wen Huang: AsiaPac Biotechnology Co., Ltd
Jiantao Ma: Chinese Academy of Sciences
Yingying Zheng: Chinese Academy of Sciences
Tzu-Ping Ko: Academia Sinica
Limin Xu: AsiaPac Biotechnology Co., Ltd
Ya-Shan Cheng: AsiaPac Biotechnology Co., Ltd
Chun-Chi Chen: Chinese Academy of Sciences
Rey-Ting Guo: Chinese Academy of Sciences

Nature Communications, 2017, vol. 8, issue 1, 1-6

Abstract: Abstract PET hydrolase (PETase), which hydrolyzes polyethylene terephthalate (PET) into soluble building blocks, provides an attractive avenue for the bioconversion of plastics. Here we present the structures of a novel PETase from the PET-consuming microbe Ideonella sakaiensis in complex with substrate and product analogs. Through structural analyses, mutagenesis, and activity measurements, a substrate-binding mode is proposed, and several features critical for catalysis are elucidated.

Date: 2017
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DOI: 10.1038/s41467-017-02255-z

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