The opposing forces of shear flow and sphingosine-1-phosphate control marginal zone B cell shuttling

Kerry Tedford (), Michael Steiner, Stanislav Koshutin, Karin Richter, Laura Tech, Yannik Eggers, Inga Jansing, Kerstin Schilling, Anja Erika Hauser, Mark Korthals and Klaus-Dieter Fischer ()
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Kerry Tedford: Otto-von-Guericke University Magdeburg
Michael Steiner: Otto-von-Guericke University Magdeburg
Stanislav Koshutin: Otto-von-Guericke University Magdeburg
Karin Richter: Otto-von-Guericke University Magdeburg
Laura Tech: Otto-von-Guericke University Magdeburg
Yannik Eggers: Otto-von-Guericke University Magdeburg
Inga Jansing: Otto-von-Guericke University Magdeburg
Kerstin Schilling: Otto-von-Guericke University Magdeburg
Anja Erika Hauser: Charite Universitätsmedizin
Mark Korthals: Otto-von-Guericke University Magdeburg
Klaus-Dieter Fischer: Otto-von-Guericke University Magdeburg

Nature Communications, 2017, vol. 8, issue 1, 1-15

Abstract: Abstract Splenic marginal zone B cells (MZB) shuttle between the blood-filled marginal zone for antigen collection and the follicle for antigen delivery. However, it is unclear how MZBs migrate directionally from the marginal zone to the follicle. Here, we show that murine MZBs migrate up shear flow via the LFA-1 (αLβ2) integrin ligand ICAM-1, but adhere or migrate down the flow via the VLA-4 integrin (α4β1) ligand VCAM-1. MZBs lacking Arhgef6 (Pak-interacting exchange factor (αPIX)) or functional LFA-1 are impaired in shuttling due to mislocalization toward the VCAM-1-rich red pulp. Sphingosine-1-phosphate (S1P) signaling through the S1PR3 receptor inhibits MZB migration up the flow, and deletion of S1pr3 in Arhgef6 −/− mice rescues mislocalized MZBs. These findings establish shear flow as a directional cue for MZB migration to the follicle, and define S1PR3 and VCAM-1 as counteracting forces that inhibit this migration.

Date: 2017
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DOI: 10.1038/s41467-017-02482-4

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