A partially-open inward-facing intermediate conformation of LeuT is associated with Na+ release and substrate transport

Daniel S. Terry, Rachel A. Kolster, Matthias Quick, Michael V. LeVine, George Khelashvili, Zhou Zhou, Harel Weinstein, Jonathan A. Javitch () and Scott C. Blanchard ()
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Daniel S. Terry: Weill Cornell Medicine
Rachel A. Kolster: Columbia University College of Physicians and Surgeons and New York State Psychiatric Institute
Matthias Quick: Columbia University College of Physicians and Surgeons and New York State Psychiatric Institute
Michael V. LeVine: Weill Cornell Medicine
George Khelashvili: Weill Cornell Medicine
Zhou Zhou: Weill Cornell Medicine
Harel Weinstein: Weill Cornell Medicine
Jonathan A. Javitch: Columbia University College of Physicians and Surgeons and New York State Psychiatric Institute
Scott C. Blanchard: Weill Cornell Medicine

Nature Communications, 2018, vol. 9, issue 1, 1-10

Abstract: Abstract Neurotransmitter:sodium symporters (NSS), targets of antidepressants and psychostimulants, clear neurotransmitters from the synaptic cleft through sodium (Na+)-coupled transport. Substrate and Na+ are thought to be transported from the extracellular to intracellular space through an alternating access mechanism by coordinated conformational rearrangements in the symporter that alternately expose the binding sites to each side of the membrane. However, the mechanism by which the binding of ligands coordinates conformational changes occurring on opposite sides of the membrane is not well understood. Here, we report the use of single-molecule fluorescence resonance energy transfer (smFRET) techniques to image transitions between distinct conformational states on both the extracellular and intracellular sides of the prokaryotic NSS LeuT, including partially open intermediates associated with transport activity. The nature and functional context of these hitherto unidentified intermediate states shed new light on the allosteric mechanism that couples substrate and Na+ symport by the NSS family through conformational dynamics.

Date: 2018
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DOI: 10.1038/s41467-017-02202-y

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