Diabetes impairs wound healing by Dnmt1-dependent dysregulation of hematopoietic stem cells differentiation towards macrophages

Jinglian Yan, Guodong Tie, Shouying Wang, Amanda Tutto, Natale DeMarco, Lyne Khair, Thomas G. Fazzio and Louis M. Messina ()
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Jinglian Yan: University of Massachusetts Medical School
Guodong Tie: University of Massachusetts Medical School
Shouying Wang: University of Massachusetts Medical School
Amanda Tutto: University of Massachusetts Medical School
Natale DeMarco: University of Massachusetts Medical School
Lyne Khair: University of Massachusetts Medical School
Thomas G. Fazzio: University of Massachusetts Medical School
Louis M. Messina: University of Massachusetts Medical School

Nature Communications, 2018, vol. 9, issue 1, 1-13

Abstract: Abstract People with type 2 diabetes mellitus (T2DM) have a 25-fold higher risk of limb loss than non-diabetics due in large part to impaired wound healing. Here, we show that the impaired wound healing phenotype found in T2D mice is recapitulated in lethally irradiated wild type recipients, whose hematopoiesis is reconstituted with hematopoietic stem cells (HSCs) from T2D mice, indicating an HSC-autonomous mechanism. This impaired wound healing phenotype of T2D mice is due to a Nox-2-dependent increase in HSC oxidant stress that decreases microRNA let-7d-3p, which, in turn, directly upregulates Dnmt1, leading to the hypermethylation of Notch1, PU.1, and Klf4. This HSC-autonomous mechanism reduces the number of wound macrophages and skews their polarization towards M1 macrophages. These findings reveal a novel inflammatory mechanism by which a metabolic disorder induces an epigenetic mechanism in HSCs, which predetermines the gene expression of terminally differentiated inflammatory cells that controls their number and function.

Date: 2018
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DOI: 10.1038/s41467-017-02425-z

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