Tuning the dynamic range of bacterial promoters regulated by ligand-inducible transcription factors

Ye Chen, Joanne M. L. Ho, David L. Shis, Chinmaya Gupta, James Long, Daniel S. Wagner, William Ott, Krešimir Josić () and Matthew R. Bennett ()
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Ye Chen: Rice University
Joanne M. L. Ho: Rice University
David L. Shis: Rice University
Chinmaya Gupta: University of Houston
James Long: Rice University
Daniel S. Wagner: Rice University
William Ott: University of Houston
Krešimir Josić: Rice University
Matthew R. Bennett: Rice University

Nature Communications, 2018, vol. 9, issue 1, 1-8

Abstract: Abstract One challenge for synthetic biologists is the predictable tuning of genetic circuit regulatory components to elicit desired outputs. Gene expression driven by ligand-inducible transcription factor systems must exhibit the correct ON and OFF characteristics: appropriate activation and leakiness in the presence and absence of inducer, respectively. However, the dynamic range of a promoter (i.e., absolute difference between ON and OFF states) is difficult to control. We report a method that tunes the dynamic range of ligand-inducible promoters to achieve desired ON and OFF characteristics. We build combinatorial sets of AraC-and LasR-regulated promoters containing −10 and −35 sites from synthetic and Escherichia coli promoters. Four sequence combinations with diverse dynamic ranges were chosen to build multi-input transcriptional logic gates regulated by two and three ligand-inducible transcription factors (LacI, TetR, AraC, XylS, RhlR, LasR, and LuxR). This work enables predictable control over the dynamic range of regulatory components.

Date: 2018
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-017-02473-5

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DOI: 10.1038/s41467-017-02473-5

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