One-step fermentative production of aromatic polyesters from glucose by metabolically engineered Escherichia coli strains

Jung Eun Yang, Si Jae Park, Won Jun Kim, Hyeong Jun Kim, Bumjoon J. Kim, Hyuk Lee, Jihoon Shin and Sang Yup Lee ()
Additional contact information
Jung Eun Yang: Korea Advanced Institute of Science and Technology (KAIST)
Si Jae Park: Ewha Womans University
Won Jun Kim: Korea Advanced Institute of Science and Technology (KAIST)
Hyeong Jun Kim: Institute for the BioCentury, KAIST
Bumjoon J. Kim: Institute for the BioCentury, KAIST
Hyuk Lee: Korea Research Institute of Chemical Technology
Jihoon Shin: Green Chemistry & Engineering Division, Korea Research Institute of Chemical Technology
Sang Yup Lee: Korea Advanced Institute of Science and Technology (KAIST)

Nature Communications, 2018, vol. 9, issue 1, 1-10

Abstract: Abstract Aromatic polyesters are widely used plastics currently produced from petroleum. Here we engineer Escherichia coli strains for the production of aromatic polyesters from glucose by one-step fermentation. When the Clostridium difficile isocaprenoyl-CoA:2-hydroxyisocaproate CoA-transferase (HadA) and evolved polyhydroxyalkanoate (PHA) synthase genes are overexpressed in a d-phenyllactate-producing strain, poly(52.3 mol% 3-hydroxybutyrate (3HB)-co-47.7 mol% d-phenyllactate) can be produced from glucose and sodium 3HB. Also, various poly(3HB-co-d-phenyllactate) polymers having 11.0, 15.8, 20.0, 70.8, and 84.5 mol% of d-phenyllactate are produced from glucose as a sole carbon source by additional expression of Ralstonia eutropha β-ketothiolase (phaA) and reductase (phaB) genes. Fed-batch culture of this engineered strain produces 13.9 g l−1 of poly(61.9 mol% 3HB-co-38.1 mol% d-phenyllactate). Furthermore, different aromatic polyesters containing d-mandelate and d-3-hydroxy-3-phenylpropionate are produced from glucose when feeding the corresponding monomers. The engineered bacterial system will be useful for one-step fermentative production of aromatic polyesters from renewable resources.

Date: 2018
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-017-02498-w Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-017-02498-w

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-017-02498-w

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().