Direct cell–cell contact between mature osteoblasts and osteoclasts dynamically controls their functions in vivo

Masayuki Furuya, Junichi Kikuta, Sayumi Fujimori, Shigeto Seno, Hiroki Maeda, Mai Shirazaki, Maki Uenaka, Hiroki Mizuno, Yoriko Iwamoto, Akito Morimoto, Kunihiko Hashimoto, Takeshi Ito, Yukihiro Isogai, Masafumi Kashii, Takashi Kaito, Shinsuke Ohba, Ung-il Chung, Alexander C. Lichtler, Kazuya Kikuchi, Hideo Matsuda, Hideki Yoshikawa and Masaru Ishii ()
Additional contact information
Masayuki Furuya: Osaka University
Junichi Kikuta: Osaka University
Sayumi Fujimori: Osaka University
Shigeto Seno: Osaka University
Hiroki Maeda: Osaka University
Mai Shirazaki: Osaka University
Maki Uenaka: Osaka University
Hiroki Mizuno: Osaka University
Yoriko Iwamoto: Osaka University
Akito Morimoto: Osaka University
Kunihiko Hashimoto: Osaka University
Takeshi Ito: Asahi Kasei Pharma Corporation
Yukihiro Isogai: Asahi Kasei Pharma Corporation
Masafumi Kashii: Osaka University
Takashi Kaito: Osaka University
Shinsuke Ohba: The University of Tokyo
Ung-il Chung: The University of Tokyo
Alexander C. Lichtler: University of Connecticut Health Center
Kazuya Kikuchi: Osaka University
Hideo Matsuda: Osaka University
Hideki Yoshikawa: Osaka University
Masaru Ishii: Osaka University

Nature Communications, 2018, vol. 9, issue 1, 1-12

Abstract: Abstract Bone homeostasis is regulated by communication between bone-forming mature osteoblasts (mOBs) and bone-resorptive mature osteoclasts (mOCs). However, the spatial–temporal relationship and mode of interaction in vivo remain elusive. Here we show, by using an intravital imaging technique, that mOB and mOC functions are regulated via direct cell–cell contact between these cell types. The mOBs and mOCs mainly occupy discrete territories in the steady state, although direct cell–cell contact is detected in spatiotemporally limited areas. In addition, a pH-sensing fluorescence probe reveals that mOCs secrete protons for bone resorption when they are not in contact with mOBs, whereas mOCs contacting mOBs are non-resorptive, suggesting that mOBs can inhibit bone resorption by direct contact. Intermittent administration of parathyroid hormone causes bone anabolic effects, which lead to a mixed distribution of mOBs and mOCs, and increase cell–cell contact. This study reveals spatiotemporal intercellular interactions between mOBs and mOCs affecting bone homeostasis in vivo.

Date: 2018
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-017-02541-w

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DOI: 10.1038/s41467-017-02541-w

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