Structure of a MacAB-like efflux pump from Streptococcus pneumoniae

Hong-Bo Yang, Wen-Tao Hou, Meng-Ting Cheng, Yong-Liang Jiang (), Yuxing Chen () and Cong-Zhao Zhou ()
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Hong-Bo Yang: University of Science and Technology of China
Wen-Tao Hou: University of Science and Technology of China
Meng-Ting Cheng: University of Science and Technology of China
Yong-Liang Jiang: University of Science and Technology of China
Yuxing Chen: University of Science and Technology of China
Cong-Zhao Zhou: University of Science and Technology of China

Nature Communications, 2018, vol. 9, issue 1, 1-11

Abstract: Abstract The spr0693-spr0694-spr0695 operon of Streptococcus pneumoniae encodes a putative ATP-binding cassette (ABC)-type efflux pump involved in the resistance of antibiotics and antimicrobial peptides. Here we report the crystal structures of Spr0694–0695 at 3.3 Å and Spr0693 at 3.0 Å resolution, revealing a MacAB-like efflux pump. The dimeric Spr0694–0695 adopts a non-canonical fold of ABC transporter, the transmembrane domain of which consists of eight tightly packed transmembrane helices with an insertion of extracellular domain between the first and second helices, whereas Spr0693 forms a nanotube channel docked onto the ABC transporter. Structural analyses combined with ATPase activity and antimicrobial susceptibility assays, enable us to propose a putative substrate-entrance tunnel with a lateral access controlled by a guard helix. Altogether, our findings provide structural insights and putative transport mechanism of a MacAB-like efflux pump in Gram-positive bacteria.

Date: 2018
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DOI: 10.1038/s41467-017-02741-4

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