Cardiogenic programming of human pluripotent stem cells by dose-controlled activation of EOMES

Martin J. Pfeiffer, Roberto Quaranta, Ilaria Piccini, Jakob Fell, Jyoti Rao, Albrecht Röpke, Guiscard Seebohm and Boris Greber ()
Additional contact information
Martin J. Pfeiffer: Max Planck Institute for Molecular Biomedicine
Roberto Quaranta: Max Planck Institute for Molecular Biomedicine
Ilaria Piccini: Max Planck Institute for Molecular Biomedicine
Jakob Fell: Max Planck Institute for Molecular Biomedicine
Jyoti Rao: Max Planck Institute for Molecular Biomedicine
Albrecht Röpke: University Hospital Münster
Guiscard Seebohm: University of Münster Medical School
Boris Greber: Max Planck Institute for Molecular Biomedicine

Nature Communications, 2018, vol. 9, issue 1, 1-8

Abstract: Abstract Master cell fate determinants are thought to induce specific cell lineages in gastrulation by orchestrating entire gene programs. The T-box transcription factor EOMES (eomesodermin) is crucially required for the development of the heart—yet it is equally important for endoderm specification suggesting that it may act in a context-dependent manner. Here, we define an unrecognized interplay between EOMES and the WNT signaling pathway in controlling cardiac induction by using loss and gain-of-function approaches in human embryonic stem cells. Dose-dependent EOMES induction alone can fully replace a cocktail of signaling molecules otherwise essential for the specification of cardiogenic mesoderm. Highly efficient cardiomyocyte programming by EOMES mechanistically involves autocrine activation of canonical WNT signaling via the WNT3 ligand, which necessitates a shutdown of this axis at a subsequent stage. Our findings provide insights into human germ layer induction and bear biotechnological potential for the robust production of cardiomyocytes from engineered stem cells.

Date: 2018
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-017-02812-6 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-017-02812-6

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-017-02812-6

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().