Leucine-rich repeat receptor-like gene screen reveals that Nicotiana RXEG1 regulates glycoside hydrolase 12 MAMP detection

Yan Wang, Yuanpeng Xu, Yujing Sun, Huibin Wang, Jiaming Qi, Bowen Wan, Wenwu Ye, Yachun Lin, Yuanyuan Shao, Suomeng Dong, Brett M. Tyler and Yuanchao Wang ()
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Yan Wang: Nanjing Agricultural University
Yuanpeng Xu: Nanjing Agricultural University
Yujing Sun: Nanjing Agricultural University
Huibin Wang: Nanjing Agricultural University
Jiaming Qi: Nanjing Agricultural University
Bowen Wan: Nanjing Agricultural University
Wenwu Ye: Nanjing Agricultural University
Yachun Lin: Nanjing Agricultural University
Yuanyuan Shao: Nanjing Agricultural University
Suomeng Dong: Nanjing Agricultural University
Brett M. Tyler: Oregon State University
Yuanchao Wang: Nanjing Agricultural University

Nature Communications, 2018, vol. 9, issue 1, 1-12

Abstract: Abstract Activation of innate immunity by membrane-localized receptors is conserved across eukaryotes. Plant genomes contain hundreds of such receptor-like genes and those encoding proteins with an extracellular leucine-rich repeat (LRR) domain represent the largest family. Here, we develop a high-throughput approach to study LRR receptor-like genes on a genome-wide scale. In total, 257 tobacco rattle virus-based constructs are generated to target 386 of the 403 identified LRR receptor-like genes in Nicotiana benthamiana for silencing. Using this toolkit, we identify the LRR receptor-like protein Response to XEG1 (RXEG1) that specifically recognizes the glycoside hydrolase 12 protein XEG1. RXEG1 associates with XEG1 via the LRR domain in the apoplast and forms a complex with the LRR receptor-like kinases BAK1 and SOBIR1 to transduce the XEG1-induced defense signal. Thus, this genome-wide silencing assay is demonstrated to be an efficient toolkit to pinpoint new immune receptors, which will contribute to developing durable disease resistance.

Date: 2018
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DOI: 10.1038/s41467-018-03010-8

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