Expansion of functional personalized cells with specific transgene combinations

Christoph Lipps, Franziska Klein, Tom Wahlicht, Virginia Seiffert, Milada Butueva, Jeannette Zauers, Theresa Truschel, Martin Luckner, Mario Köster, Roderick MacLeod, Jörn Pezoldt, Jochen Hühn, Qinggong Yuan, Peter Paul Müller, Henning Kempf, Robert Zweigerdt, Oliver Dittrich-Breiholz, Thomas Pufe, Rainer Beckmann, Wolf Drescher, Jose Riancho, Carolina Sañudo, Thomas Korff, Bertram Opalka, Vera Rebmann, Joachim R. Göthert, Paula M. Alves, Michael Ott, Roland Schucht, Hansjörg Hauser, Dagmar Wirth () and Tobias May ()
Additional contact information
Christoph Lipps: HZI – Helmholtz Centre for Infection Research
Franziska Klein: HZI – Helmholtz Centre for Infection Research
Tom Wahlicht: HZI – Helmholtz Centre for Infection Research
Virginia Seiffert: HZI – Helmholtz Centre for Infection Research
Milada Butueva: HZI – Helmholtz Centre for Infection Research
Jeannette Zauers: InSCREENeX GmbH
Theresa Truschel: InSCREENeX GmbH
Martin Luckner: InSCREENeX GmbH
Mario Köster: HZI – Helmholtz Centre for Infection Research
Roderick MacLeod: Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures
Jörn Pezoldt: HZI – Helmholtz Centre for Infection Research
Jochen Hühn: HZI – Helmholtz Centre for Infection Research
Qinggong Yuan: Hannover Medical School
Peter Paul Müller: HZI – Helmholtz Centre for Infection Research
Henning Kempf: Hannover Medical School, MHH
Robert Zweigerdt: Hannover Medical School, MHH
Oliver Dittrich-Breiholz: Medical School Hannover
Thomas Pufe: RWTH Aachen University
Rainer Beckmann: RWTH Aachen University
Wolf Drescher: Aachen University Hospital, RWTH Aachen University
Jose Riancho: Hospital U.M. Valdecilla, University of Cantabria, IDIVAL
Carolina Sañudo: Hospital U.M. Valdecilla, University of Cantabria, IDIVAL
Thomas Korff: University Heidelberg
Bertram Opalka: University Hospital Essen
Vera Rebmann: University Hospital Essen
Joachim R. Göthert: University Hospital Essen
Paula M. Alves: Universidade Nova de Lisboa
Michael Ott: Hannover Medical School
Roland Schucht: InSCREENeX GmbH
Hansjörg Hauser: HZI – Helmholtz Centre for Infection Research
Dagmar Wirth: HZI – Helmholtz Centre for Infection Research
Tobias May: InSCREENeX GmbH

Nature Communications, 2018, vol. 9, issue 1, 1-12

Abstract: Abstract Fundamental research and drug development for personalized medicine necessitates cell cultures from defined genetic backgrounds. However, providing sufficient numbers of authentic cells from individuals poses a challenge. Here, we present a new strategy for rapid cell expansion that overcomes current limitations. Using a small gene library, we expanded primary cells from different tissues, donors, and species. Cell-type-specific regimens that allow the reproducible creation of cell lines were identified. In depth characterization of a series of endothelial and hepatocytic cell lines confirmed phenotypic stability and functionality. Applying this technology enables rapid, efficient, and reliable production of unlimited numbers of personalized cells. As such, these cell systems support mechanistic studies, epidemiological research, and tailored drug development.

Date: 2018
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DOI: 10.1038/s41467-018-03408-4

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