The preprophase band-associated kinesin-14 OsKCH2 is a processive minus-end-directed microtubule motor

Kuo-Fu Tseng, Pan Wang, Yuh-Ru Julie Lee, Joel Bowen, Allison M. Gicking, Lijun Guo, Bo Liu () and Weihong Qiu ()
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Kuo-Fu Tseng: Oregon State University
Pan Wang: Oregon State University
Yuh-Ru Julie Lee: University of California at Davis
Joel Bowen: Oregon State University
Allison M. Gicking: Oregon State University
Lijun Guo: Henan University
Bo Liu: University of California at Davis
Weihong Qiu: Oregon State University

Nature Communications, 2018, vol. 9, issue 1, 1-11

Abstract: Abstract In animals and fungi, cytoplasmic dynein is a processive minus-end-directed motor that plays dominant roles in various intracellular processes. In contrast, land plants lack cytoplasmic dynein but contain many minus-end-directed kinesin-14s. No plant kinesin-14 is known to produce processive motility as a homodimer. OsKCH2 is a plant-specific kinesin-14 with an N-terminal actin-binding domain and a central motor domain flanked by two predicted coiled-coils (CC1 and CC2). Here, we show that OsKCH2 specifically decorates preprophase band microtubules in vivo and transports actin filaments along microtubules in vitro. Importantly, OsKCH2 exhibits processive minus-end-directed motility on single microtubules as individual homodimers. We find that CC1, but not CC2, forms the coiled-coil to enable OsKCH2 dimerization. Instead, our results reveal that removing CC2 renders OsKCH2 a nonprocessive motor. Collectively, these results show that land plants have evolved unconventional kinesin-14 homodimers with inherent minus-end-directed processivity that may function to compensate for the loss of cytoplasmic dynein.

Date: 2018
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DOI: 10.1038/s41467-018-03480-w

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